diff --git a/instrumentation/opentelemetry-instrumentation-django/src/opentelemetry/instrumentation/django/middleware/sqlcommenter_middleware.py b/instrumentation/opentelemetry-instrumentation-django/src/opentelemetry/instrumentation/django/middleware/sqlcommenter_middleware.py
index 89d8a9b776..30492a8be5 100644
--- a/instrumentation/opentelemetry-instrumentation-django/src/opentelemetry/instrumentation/django/middleware/sqlcommenter_middleware.py
+++ b/instrumentation/opentelemetry-instrumentation-django/src/opentelemetry/instrumentation/django/middleware/sqlcommenter_middleware.py
@@ -1,5 +1,3 @@
-#!/usr/bin/python
-#
 # Copyright The OpenTelemetry Authors
 #
 # Licensed under the Apache License, Version 2.0 (the "License");
diff --git a/opentelemetry-instrumentation/src/opentelemetry/instrumentation/auto_instrumentation/__init__.py b/opentelemetry-instrumentation/src/opentelemetry/instrumentation/auto_instrumentation/__init__.py
index 5758ef1834..a09334432d 100644
--- a/opentelemetry-instrumentation/src/opentelemetry/instrumentation/auto_instrumentation/__init__.py
+++ b/opentelemetry-instrumentation/src/opentelemetry/instrumentation/auto_instrumentation/__init__.py
@@ -1,5 +1,3 @@
-#!/usr/bin/env python3
-
 # Copyright The OpenTelemetry Authors
 #
 # Licensed under the Apache License, Version 2.0 (the "License");
diff --git a/opentelemetry-instrumentation/src/opentelemetry/instrumentation/bootstrap.py b/opentelemetry-instrumentation/src/opentelemetry/instrumentation/bootstrap.py
index afd5a045d9..0c8f0aa3c4 100644
--- a/opentelemetry-instrumentation/src/opentelemetry/instrumentation/bootstrap.py
+++ b/opentelemetry-instrumentation/src/opentelemetry/instrumentation/bootstrap.py
@@ -1,5 +1,3 @@
-#!/usr/bin/env python3
-
 # Copyright The OpenTelemetry Authors
 #
 # Licensed under the Apache License, Version 2.0 (the "License");