diff --git a/instrumentation/opentelemetry-instrumentation-django/src/opentelemetry/instrumentation/django/__init__.py b/instrumentation/opentelemetry-instrumentation-django/src/opentelemetry/instrumentation/django/__init__.py
index d37c45993c..facc2e4176 100644
--- a/instrumentation/opentelemetry-instrumentation-django/src/opentelemetry/instrumentation/django/__init__.py
+++ b/instrumentation/opentelemetry-instrumentation-django/src/opentelemetry/instrumentation/django/__init__.py
@@ -251,7 +251,7 @@ def response_hook(span, request, response):
     _report_old,
 )
 from opentelemetry.instrumentation.django.environment_variables import (
-    OTEL_PYTHON_DJANGO_INSTRUMENT,
+    OTEL_PYTHON_DJANGO_INSTRUMENT, OTEL_PYTHON_DJANGO_MIDDLEWARE_INDEX
 )
 from opentelemetry.instrumentation.django.middleware.otel_middleware import (
     _DjangoMiddleware,
@@ -388,10 +388,11 @@ def _instrument(self, **kwargs):
 
         is_sql_commentor_enabled = kwargs.pop("is_sql_commentor_enabled", None)
 
+        opentelemetry_middleware_index = environ.get(OTEL_PYTHON_DJANGO_MIDDLEWARE_INDEX, 0)
         if is_sql_commentor_enabled:
-            settings_middleware.insert(0, self._sql_commenter_middleware)
+            settings_middleware.insert(opentelemetry_middleware_index, self._sql_commenter_middleware)
 
-        settings_middleware.insert(0, self._opentelemetry_middleware)
+        settings_middleware.insert(opentelemetry_middleware_index, self._opentelemetry_middleware)
 
         setattr(settings, _middleware_setting, settings_middleware)
 
diff --git a/instrumentation/opentelemetry-instrumentation-django/src/opentelemetry/instrumentation/django/environment_variables.py b/instrumentation/opentelemetry-instrumentation-django/src/opentelemetry/instrumentation/django/environment_variables.py
index 4972a62e93..3034e03d21 100644
--- a/instrumentation/opentelemetry-instrumentation-django/src/opentelemetry/instrumentation/django/environment_variables.py
+++ b/instrumentation/opentelemetry-instrumentation-django/src/opentelemetry/instrumentation/django/environment_variables.py
@@ -13,3 +13,4 @@
 # limitations under the License.
 
 OTEL_PYTHON_DJANGO_INSTRUMENT = "OTEL_PYTHON_DJANGO_INSTRUMENT"
+OTEL_PYTHON_DJANGO_MIDDLEWARE_INDEX = "OTEL_PYTHON_DJANGO_MIDDLEWARE_INDEX"